Publications

PAPERS

2021


Alessandro Porro, Gerardo Abbandonato, Valentina Veronesi, Alberto Russo, Anna Binda, Laura Antolini, Tiziana Granata, Barbara Castellotti, Carla Marini, Anna Moroni, Jacopo C DiFrancesco, Ilaria Rivolta.
Do the functional properties of HCN1 mutants correlate with the clinical features in epileptic patients?
Prog Biophys Mol Biol. 2021 Jul 23;S0079-6107(21)00089-4. doi: 10.1016/j.pbiomolbio.2021.07.008
https://www.sciencedirect.com/science/article/abs/pii/S0079610721000894

Andrea Saponaro, Daniel Bauer, M Hunter Giese, Paolo Swuec, Alessandro Porro, Federica Gasparri, Atiyeh Sadat Sharifzadeh, Antonio Chaves-Sanjuan, Laura Alberio, Giacomo Parisi, Gabriele Cerutti, Oliver B Clarke, Kay Hamacher, Henry M Colecraft, Filippo Mancia, Wayne A Hendrickson, Steven A Siegelbaum, Dario DiFrancesco, Martino Bolognesi, Gerhard Thiel, Bina Santoro, Anna Moroni.
Gating movements and ion permeation in HCN4 pacemaker channels.
Mol Cell. 2021 Jul 15;81(14):2929-2943.e6. doi: 10.1016/j.molcel.2021.05.033.
https://www.cell.com/molecular-cell/fulltext/S1097-2765(21)00446-9

Anja J Engel, Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L Van Etten, Anna Moroni, Gerhard Thiel.
Codon Bias Can Determine Sorting of a Potassium Channel Protein.
Cells. 2021 May 7;10(5):1128. doi: 10.3390/cells10051128.
https://www.mdpi.com/2073-4409/10/5/1128

Andrea Saponaro, Atiyeh Sadat Sharifzadeh, Anna Moroni.
Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography.
Methods Enzymol. 2021;652:105-123. doi: 10.1016/bs.mie.2021.01.043.
https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(21)00065-3


2020


Stefanie Scheiper-Welling, Paolo Zuccolini, Oliver Rauh, Britt-Maria Beckmann, Christof Geisen, Anna Moroni, Gerhard Thiel, Silke Kauferstein.
Characterization of an N-terminal Na v 1.5 channel variant – a potential risk factor for arrhythmias and sudden death?
BMC Med Genet. 2020 Nov 19;21(1):227. doi: 10.1186/s12881-020-01170-3.
https://bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-020-01170-3

Kerri Kukovetz, Brigitte Hertel, Christopher R Schvarcz, Andrea Saponaro, Mirja Manthey, Ulrike Burk, Timo Greiner, Grieg F Steward, James L Van Etten, Anna Moroni, Gerhard Thiel, Oliver Rauh.
A Functional K + Channel from Tetraselmis Virus 1, a Member of the Mimiviridae.
Viruses. 2020 Sep 29;12(10):1107. doi: 10.3390/v12101107.
https://www.mdpi.com/1999-4915/12/10/1107

Alessandro Porro, Anna Binda, Matteo Pisoni, Chiara Donadoni, Ilaria Rivolta, Andrea Saponaro.
Rational design of a mutation to investigate the role of the brain protein TRIP8b in limiting the cAMP response of HCN channels in neurons.
J Gen Physiol. 2020 Sep 7;152(9):e202012596. doi: 10.1085/jgp.202012596.
https://rupress.org/jgp/article-lookup/doi/10.1085/jgp.202012596

Alessandro Porro, Gerhard Thiel, Anna Moroni, Andrea Saponaro.
cyclic AMP Regulation and Its Command in the Pacemaker Channel HCN4.
Front Physiol. 2020 Jul 7;11:771. doi: 10.3389/fphys.2020.00771. eCollection 2020.
https://www.frontiersin.org/articles/10.3389/fphys.2020.00771/full

Janine D Brunner, Roman P Jakob, Tobias Schulze, Yvonne Neldner, Anna Moroni, Gerhard Thiel, Timm Maier, Stephan Schenck.
Structural basis for ion selectivity in TMEM175 K + channels.
Elife. 2020 Apr 8;9:e53683. doi: 10.7554/eLife.53683.
https://doi.org/10.7554/eLife.53683


2019


Denise Eckert, Tobias Schulze, Julian Stahl, Oliver Rauh, James L Van Etten, Brigitte Hertel, Indra Schroeder, Anna Moroni, Gerhard Thiel.
A small viral potassium ion channel with an inherent inward rectification.
Channels (Austin). 2019 Dec;13(1):124-135. doi: 10.1080/19336950.2019.1605813.
https://www.tandfonline.com/doi/full/10.1080/19336950.2019.1605813

Alessandro Porro, Andrea Saponaro, Federica Gasparri, Daniel Bauer, Christine Gross, Matteo Pisoni, Gerardo Abbandonato, Kay Hamacher, Bina Santoro, Gerhard Thiel, Anna Moroni.
The HCN domain couples voltage gating and cAMP response in hyperpolarization-activated cyclic nucleotide-gated channels.
Elife. 2019 Nov 26;8:e49672. doi: 10.7554/eLife.49672.
https://doi.org/10.7554/eLife.49672


2018


Alberio L, Locarno A, Saponaro A, Romano E, Bercier V, Albadri S, Simeoni F, Moleri S, Pelucchi S, Porro A, Marcello E, Barsotti N, Kukovetz K, Boender AJ, Contestabile A, Luo S, Moutal A, Ji Y, Romani G, Beltrame M, Del Bene F, Di Luca M, Khanna R, Colecraft HM, Pasqualetti M, Thiel G, Tonini R, Moroni A.
A light-gated potassium channel for sustained neuronal inhibition.
Nat Methods.. 2018 Nov;15(11):969-976. doi: 10.1038/s41592-018-0186-9.
https://www.nature.com/articles/s41592-018-0186-9

Carla Marini, Alessandro Porro, Agnès Rastetter, Carine Dalle, Ilaria Rivolta, Daniel Bauer, Renske Oegema, Caroline Nava, Elena Parrini, Davide Mei, Catherine Mercer, Radhika Dhamija, Chelsea Chambers, Christine Coubes, Julien Thévenon, Paul Kuentz, Sophie Julia, Laurent Pasquier, Christèle Dubourg, Wilfrid Carré, Anna Rosati, Federico Melani, Tiziana Pisano, Maria Giardino, A Micheil Innes, Yves Alembik, Sophie Scheidecker, Manuela Santos, Sonia Figueiroa, Cristina Garrido, Carlo Fusco, Daniele Frattini, Carlotta Spagnoli, Anna Binda, Tiziana Granata, Francesca Ragona, Elena Freri, Silvana Franceschetti, Laura Canafoglia, Barbara Castellotti, Cinzia Gellera, Raffaella Milanesi, Maria Margherita Mancardi, Damien R Clark, Fernando Kok, Katherine L Helbig, Shoji Ichikawa, Laurie Sadler, Jana Neupauerová, Petra Laššuthova, Katalin Šterbová, Annick Laridon, Eva Brilstra, Bobby Koeleman, Johannes R Lemke, Federico Zara, Pasquale Striano, Julie Soblet, Guillaume Smits, Nicolas Deconinck, Andrea Barbuti,
Dario DiFrancesco, Eric LeGuern, Renzo Guerrini, Bina Santoro, Kay Hamacher, Gerhard Thiel, Anna Moroni, Jacopo C DiFrancesco, Christel Depienne.
HCN1 mutation spectrum: from neonatal epileptic encephalopathy to benign generalized epilepsy and beyond.
Brain. 2018 Nov 1;141(11):3160-3178. doi: 10.1093/brain/awy263.
https://academic.oup.com/brain/article-lookup/doi/10.1093/brain/awy263

Greiner T, Moroni A, Van Etten JL, Thiel G.
Genes for Membrane Transport Proteins: Not So Rare in Viruses.
Viruses. 2018 Aug 26;10(9). pii: E456. doi: 10.3390/v10090456.
https://www.mdpi.com/1999-4915/10/9/456

Gross C, Saponaro A, Santoro B, Moroni A, Thiel G, Hamacher K.
Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide-gated cation channel.
J Biol Chem. 2018 Aug 17;293(33):12908-12918. doi: 10.1074/jbc.RA118.002139.
http://www.jbc.org/cgi/pmidlookup?view=long&pmid=29936413

Saponaro A, Cantini F, Porro A, Bucchi A, DiFrancesco D, Maione V, Donadoni C, Introini B, Mesirca P, Mangoni ME, Thiel G, Banci L, Santoro B, Moroni A.
A synthetic peptide that prevents cAMP regulation in mammalian hyperpolarization-activated cyclic nucleotide-gated (HCN) channels.
Elife. 2018 Jun 20;7. pii: e35753. doi: 10.7554/eLife.35753.
https://doi.org/10.7554/eLife.35753

Bjarnsholt T, Buhlin K, Dufrêne YF, Gomelsky M, Moroni A, Ramstedt M, Rumbaugh KP, Schulte T, Sun L, Åkerlund B, Römling U.
Biofilm formation – what we can learn from recent developments.
J Intern Med. 2018 Oct;284(4):332-345. doi: 10.1111/joim.12782.
https://doi.org/10.1111/joim.12782

Voos P, Fuck S, Weipert F, Babel L, Tandl D, Meckel T, Hehlgans S, Fournier C, Moroni A, Rödel F, Thiel G.
Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.
Front Immunol. 2018 Apr 30;9:922. doi: 10.3389/fimmu.2018.00922. eCollection 2018.
https://dx.doi.org/10.3389/fimmu.2018.00922

Rondelli V, Del Favero E, Brocca P, Fragneto G, Trapp M, Mauri L, Ciampa MG, Romani G, Braun CJ, Winterstein L, Schroeder I, Thiel G, Moroni A, Cantu’ L.
Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform.
Biochim Biophys Acta Gen Subj. 2018 Aug;1862(8):1742-1750. doi: 10.1016/j.bbagen.2018.05.007.
https://linkinghub.elsevier.com/retrieve/pii/S0304-4165(18)30139-9

Winterstein LM, Kukovetz K, Rauh O, Turman DL, Braun C, Moroni A, Schroeder I, Thiel G.
Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers.
J Gen Physiol. 2018 Apr 2;150(4):637-646. doi: 10.1085/jgp.201711904.
http://jgp.rupress.org/cgi/pmidlookup?view=long&pmid=29487088

Servatius H, Porro A, Pless SA, Schaller A, Asatryan B, Tanner H, de Marchi SF, Roten L, Seiler J, Haeberlin A, Baldinger SH, Noti F, Lam A, Fuhrer J, Moroni A, Medeiros-Domingo A.
Phenotypic Spectrum of HCN4 Mutations: A Clinical Case.
Circ Genom Precis Med. 2018 Feb;11(2):e002033. doi: 10.1161/CIRCGEN.117.002033.
https://www.ncbi.nlm.nih.gov/pubmed/29440115


2017

Rauh O, Hansen UP, Mach S, Hartel AJW, Shepard KL, Thiel G, Schroeder I. Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter. FEBS Lett. 2017 Dec;591(23):3850-3860. doi: 10.1002/1873-3468.12898.
http://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12898/abstract

Saponaro A, Porro A, Chaves-Sanjuan A, Nardini M, Rauh O, Thiel G, Moroni A. Fusicoccin Activates KAT1 Channels by Stabilizing Their Interaction with 14-3-3 Proteins. Plant Cell. 2017 Oct;29(10):2570-2580. doi: 10.1105/tpc.17.00375.
http://www.plantcell.org/content/29/10/2570.long

Rauh O, Urban M, Henkes LM, Winterstein T, Greiner T, Van Etten JL, Moroni A, Kast SM, Thiel G, Schroeder I. Identification of Intrahelical Bifurcated H-Bonds as a New Type of Gate in K+ Channels. J Am Chem Soc. 2017 Jun 7;139(22):7494-7503. doi: 10.1021/jacs.7b01158.
https://pubs.acs.org/doi/10.1021/jacs.7b01158

Cosentino C, Alberio L, Thiel G, Moroni A. Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels. Methods Mol Biol. 2017;1596:271-285. doi: 10.1007/978-1-4939-6940-1_17.
https://link.springer.com/protocol/10.1007%2F978-1-4939-6940-1_17

Weißgraeber S, Saponaro A, Thiel G, Hamacher K. A reduced mechanical model for cAMP-modulated gating in HCN channels. Sci Rep. 2017 Jan 11;7:40168. doi: 10.1038/srep40168.
https://www.nature.com/articles/srep40168